Coding

Part:BBa_K863012:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-18)

tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 990
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 818


Design Notes

  • PCR on BBa_K863011 with primers with BioBrick Prefix in the fwd primer and Suffix and His-tag in the rev primer.
    • fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCTGGCGCGCAGGAGCTT-3'
    • rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGACCCACCTCGAGGACTC-3'

Source

The gene sequence was isolated from Thermus thermophilus HB27.

References

Miyazaki, K. (2005). "A hyperthermophilic laccase from Thermus thermophilus HB27." Extremophiles 9(6): 415-425.